Some of the steps involved in the production of Humulin are given below. Choose the correct sequence.

(i) synthesis of gene (DNA) for human insulin artificially (ii) culturing transformed E.coli in bioreactors. (iii) purification of humulin. (iv) insertion of human insulin gene into plasmid. (v) introduction of recombinant plasmid into E.coli. (vi) extraction of recombinant gene product from E.coli