Plasmid pBR322 has Pst I restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactoside production and the recombinant plasmid is inserted in an E.coli strain

Question

Plasmid pBR322 has Pst I restriction enzyme site within gene amp^R that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactoside production and the recombinant plasmid is inserted in an E.coli strain

It will be able to produce a novel protein with dual ability

It will not be able to confer ampicillin resistance to the host cell

The transformed cells will have the ability to resist ampicillin as well as produce $\beta$-galactoside

It will lead to lysis of host cell

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